bio scale mini unosphere supra affinity chromatography cartridges  (Bio-Rad)

Journal: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology

Article Title: Blocking function of allergen-specific immunoglobulin G, F(ab') 2 , and Fab antibodies prepared from patients undergoing Dermatophagoides pteronyssinus immunotherapy.

doi: 10.1016/j.anai.2022.03.031

Figure Lengend Snippet: Figure 1. Flowchart of the study design. Procedure for purification of the total IgG antibodies from serum samples by affinity chromatography and the preparation and functional investigation of the sF(ab')2 and sFab antibodies. BATI, basophil activation inhibition assay; CRIA, cytokine release inhibition assay; Der-p, Dermatophagoides pteronyssinus; FceR I, high-affinity IgE receptors; FceR II, low-affinity IgE receptors; FITC, fluorescein isothiocyanate; IgG, immunoglobulin G; IgE-FAB, IgE-facilitated allergen binding to B-cells; SCIT, sub- cutaneous allergen immunotherapy; sIgG, specific immunoglobulin G; sIgE, specific immunoglobulin E.

Article Snippet: Purification of Allergen-Specific Immunoglobulin G Antibodies For purification of total IgG, the serum samples from SCIT patients at weeks 0 and 156 (2 mL, diluted 1:10 in binding buffer) were loaded into affinity chromatography columns (Bio-Scale Mini UNOsphere SUPrA Protein-A cartridges; Bio-Rad, Hercules, California), previously equilibrated with binding buffer (0.02 M sodium phosphate, 0.02 M sodium citrate, pH 7.5).

Techniques: Chromatography, Functional Assay, Activation Assay, Inhibition, Binding Assay